Nadeau Owen W, Carlson Gerald M
CSH Protoc. 2007 Apr 1;2007:pdb.prot4587. doi: 10.1101/pdb.prot4587.
INTRODUCTIONThis protocol describes a method for chemical cross-linking of proteins using sulfo-MBS (m-maleimidobenzoyl-N-hydroxysulfo-succinimide ester). Optimal conditions for cross-linking can be determined rapidly for a fixed concentration of a protein complex by varying the time of cross-linking, pH of the reaction, and concentration of sulfo-MBS. Typically, these screens require only small amounts of target proteins and can be carried out in less than a day.
引言
本方案描述了一种使用磺基-MBS（间马来酰亚胺苯甲酰-N-羟基磺基琥珀酰亚胺酯）对蛋白质进行化学交联的方法。通过改变交联时间、反应pH值和磺基-MBS的浓度，可以快速确定固定浓度蛋白质复合物的最佳交联条件。通常，这些筛选仅需要少量的目标蛋白质，并且可以在不到一天的时间内完成。
