Sive Hazel L, Grainger Robert M, Harland Richard M
CSH Protoc. 2007 May 1;2007:pdb.top8. doi: 10.1101/pdb.top8.
INTRODUCTIONGood animal husbandry is vital for maintaining a healthy frog population. This requires some effort but is generally rewarded by high-quality egg and embryo production. A healthy frog is placid, with moderately slimy skin and a nice pear shape. Jumpy frogs, frogs with dry or excessively slimy skin, bloated frogs, and frogs that look gray and thin or reddish are not healthy and should not be used for egg collection, as this would lead to further deterioration of the animals' condition, and the resulting eggs would be generally unsuitable for experimental purposes. This article describes proper husbandry techniques for Xenopus laevis in the laboratory.
引言
良好的动物饲养管理对于维持健康的青蛙种群至关重要。这需要付出一些努力,但通常会换来高质量的卵和胚胎产出。健康的青蛙性情温顺,皮肤适度黏滑,体型呈优美的梨形。跳跃活跃的青蛙、皮肤干燥或过于黏滑的青蛙、腹部肿胀的青蛙以及看起来灰暗消瘦或发红的青蛙都不健康,不应被用于采集卵,因为这会导致动物状况进一步恶化,而且所产出的卵通常不适用于实验目的。本文介绍了在实验室中对非洲爪蟾进行妥善饲养管理的技术。