果蝇胚胎去卵壳处理
Drosophila embryo dechorionation.
作者信息
Rothwell Wendy F, Sullivan William
出版信息
CSH Protoc. 2007 Sep 1;2007:pdb.prot4826. doi: 10.1101/pdb.prot4826.
PMID:21357166
Abstract
INTRODUCTIONEarly Drosophila embryos are particularly amenable to cellular analysis. However, they are protected by an outer chorion layer, as well as an impermeable and opaque vitelline membrane. Consequently, preparation of Drosophila embryos for fixed-sample analysis must include procedures for chorion removal, vitelline membrane permeabilization, fixation, and finally, vitelline membrane removal. This protocol describes a method for removing the chorion layer.
摘要
引言
早期果蝇胚胎特别适合进行细胞分析。然而，它们受到外层卵壳层以及不透水且不透明的卵黄膜的保护。因此，制备用于固定样本分析的果蝇胚胎必须包括去除卵壳、使卵黄膜通透、固定以及最后去除卵黄膜的步骤。本方案描述了一种去除卵壳层的方法。
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