镧（III）对嗜热四膜虫金属硫蛋白 MTT1 和 MTT2 的影响。
Lanthanum(III) impacts on metallothionein MTT1 and MTT2 from Tetrahymena thermophila.
机构信息
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, 92 Wucheng Rd, Taiyuan, 030006, People's Republic of China.
出版信息
Biol Trace Elem Res. 2011 Dec;143(3):1808-18. doi: 10.1007/s12011-011-9004-2. Epub 2011 Feb 26.
Metallothionein MTT1 and MTT2 from Tetrahymena thermophila are sulfydryl-rich proteins that can bind to and are inducible by heavy metals such as mercury, cadmium, zinc, and copper. However, little is known about the induction and binding of T. thermophila metallothionein by trivalent metals. In this study, we found that 10-80 μM La(3+) can promote Tetrahymena cells proliferation, and fluorescence spectrum analysis showed that La(3+) can enter T. thermophila cells. Real-time quantitative polymerase chain reaction showed La(3+) induced the expression of MTT1 and MTT2. Furthermore, Fluorescence analysis indicated La(3+) bind to MTT1 and MTT2. These results implied that La(3+) could interact with MTT1 and MTT2 via aspartic or glutamic acid oxygen atoms.
热聚生菌金属硫蛋白 MTT1 和 MTT2 富含巯基，可与汞、镉、锌、铜等重金属结合，并可诱导这些重金属结合。然而，关于三价金属对热聚生菌金属硫蛋白的诱导和结合知之甚少。在这项研究中，我们发现 10-80μM 的 La(3+) 可以促进四膜虫细胞的增殖，荧光光谱分析表明 La(3+) 可以进入热聚生菌细胞。实时定量聚合酶链反应显示 La(3+) 诱导 MTT1 和 MTT2 的表达。此外，荧光分析表明 La(3+) 与 MTT1 和 MTT2 结合。这些结果表明 La(3+) 可以通过天冬氨酸或谷氨酸的氧原子与 MTT1 和 MTT2 相互作用。
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