Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
J Mater Sci Mater Med. 2011 Apr;22(4):969-76. doi: 10.1007/s10856-011-4266-y. Epub 2011 Mar 1.
It is crucial to measure the mechanical function of regenerating bone in order to assess the mechanical performance of the regenerating portion as well as the efficiency of the regeneration methods. In this study, nanoindentation was applied to regenerating and intact rabbit ulnae to determine the material properties of hardness and elasticity; viscoelasticity was also investigated to precisely evaluate the material properties. Both intact and regenerating bones exhibited remarkable viscoelasticity manifested as a creep behavior during load hold at the maximum load, and the creep was significantly greater in the regenerating bone than the intact bone. The creep resulted in an overestimation of the hardness and Young's modulus. Hence, during nanoindentation testing of bones, the effect of creep should be eliminated. Moreover, the regenerating bone had lower hardness and Young's modulus than the intact bone. The nanoindentation technique proved to be a powerful approach for understanding the mechanical properties of regenerating bone.
为了评估再生部分的机械性能和再生方法的效率，测量再生骨的机械功能至关重要。在这项研究中，纳米压痕法被应用于再生和完整的兔尺骨，以确定硬度和弹性等材料性能；还研究了粘弹性，以精确评估材料性能。完整和再生的骨骼都表现出显著的粘弹性，表现为在最大载荷下保持载荷时的蠕变行为，而在再生骨中的蠕变明显大于完整骨中的蠕变。蠕变导致硬度和杨氏模量的高估。因此，在骨骼的纳米压痕测试中，应消除蠕变的影响。此外，再生骨的硬度和杨氏模量低于完整骨。纳米压痕技术被证明是一种理解再生骨机械性能的有力方法。
