Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, South Korea.
Cell Mol Neurobiol. 2011 Jul;31(5):729-36. doi: 10.1007/s10571-011-9670-9. Epub 2011 Mar 1.
In this study, we observed neuroblast differentiation in the somatosensory cortex (SSC) and hippocampal CA1 region (CA1), which is vulnerable to oxidative stress, of the mouse at various early postnatal days (P) 1, 7, 14, and 21 using doublecortin (DCX, a marker for neuroblasts). Cresyl violet and NeuN (Neuronal Nuclei) staining showed development of layers as well as neurons in the SSC and CA1. At P1, DCX-positive neuroblasts expressed strong DCX immunoreactivity in both the SSC and CA1. Thereafter, DCX immunoreactivity was decreased with time. At P7, many DCX-immunoreactive neuroblasts were well detected in the SSC and CA1. At P14, some DCX-positive neuroblasts were found in the SSC and CA1: The immunoreactivity was weak. At P21, DCX immunoreactivity was hardly found in cells in the SSC and CA1. These results suggest that DCX-positive neuroblasts were significantly decreased in the mouse SSC and CA1 from P14.
在这项研究中，我们使用双皮质素 (DCX，神经前体细胞的标志物) 观察了小鼠在出生后不同时间点（P1、7、14 和 21）的体感皮层 (SSC) 和海马 CA1 区 (CA1) 中的神经细胞分化情况，这两个区域容易受到氧化应激的影响。Cresyl 紫和 NeuN (神经元核) 染色显示了 SSC 和 CA1 中各层的发育和神经元。在 P1 时，SSC 和 CA1 中的 DCX 阳性神经前体细胞表达强烈的 DCX 免疫反应性。此后，随着时间的推移，DCX 免疫反应性逐渐降低。在 P7 时，SSC 和 CA1 中可以很好地检测到许多 DCX 免疫阳性的神经前体细胞。在 P14 时，SSC 和 CA1 中仍存在少量的 DCX 阳性神经前体细胞，但免疫反应性较弱。在 P21 时，SSC 和 CA1 中的 DCX 免疫反应性几乎无法检测到。这些结果表明，从 P14 开始，小鼠 SSC 和 CA1 中的 DCX 阳性神经前体细胞显著减少。
