Department of Histology-Embryology and Anthropology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Histol Histopathol. 2011 Apr;26(4):421-6. doi: 10.14670/HH-26.421.
To investigate the expression of HIF-1α during the postnatal development of the mandibular condyle under normal and soft consistency diet conditions.
Forty eight Wistar-Furth rats, aged 21 days, were divided into two groups, each being fed with either normal or soft consistency diet. Three animals from each group were sacrificed after 3, 7, 10 days (initial period), 14, 17 days (intermediate period), and 21, 24 and 28 days (final period) after the start of the experiment. Immediately after sacrifice, the mandible was excised surgically, fixed, demineralised and embedded in paraffin. Six µm thick sections were obtained, processed for conventional and immunohistochemical staining and observed in the optical microscope. HIF-1α expression was assessed semiquantitavely and graded separately for the nucleus stained cells and the cells stained exclusively in the cytoplasm. Differences in HIF-1α expression in the experimental groups were evaluated statistically.
HIF-1α expression was evident in the proliferative and chondroblast layers. No differences were observed between the anterior, the intermediate and the posterior parts of the condylar cartilage. In the normal consistency diet group, nuclear HIF-1α expression increased gradually until the end of the experiment. On the contrary, in the soft diet animals, nuclear HIF-1α expression increased only at the final period of the experiment. The normal diet fed animals exhibited more intense nuclear HIF-1α expression compared to cytoplasmic expression.
HIF-1α expression in condylar chondrocytes varies under altered conditions of diet consistency.
研究在正常和软食两种饮食条件下，下颌骨髁突在出生后发育过程中 HIF-1α 的表达情况。
48 只 21 天大的 Wistar-Furth 大鼠分为两组，分别给予正常或软食。每组各有 3 只动物在实验开始后 3、7、10 天（初始期）、14、17 天（中期）、21、24 和 28 天（末期）被处死。处死即刻，手术切除下颌骨，固定、脱钙、石蜡包埋。获取 6μm 厚的切片，进行常规和免疫组织化学染色，并在光学显微镜下观察。通过半定量评估 HIF-1α 表达，并分别对核染色细胞和仅细胞质染色细胞进行分级。通过统计学方法评估实验组之间 HIF-1α 表达的差异。
HIF-1α 在增殖层和软骨细胞层均有表达。髁突软骨的前、中、后三部分之间无差异。在正常饮食组中，核 HIF-1α 表达逐渐增加，直至实验结束。相反，在软食组中，核 HIF-1α 表达仅在实验末期增加。正常饮食组的核 HIF-1α 表达比细胞质表达更为强烈。
在饮食一致性改变的情况下，髁状突软骨细胞中的 HIF-1α 表达发生变化。
