State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
J Biomed Nanotechnol. 2010 Dec;6(6):658-66. doi: 10.1166/jbn.2010.1159.
In this study, we developed a method to obtain high surface area nanofiber meshes composed of chitosan of a number of molecular weights. This method required decreasing the viscosity and surface tension of the chitosan solution as well as optimization of the electrospinning parameters such as applied voltage and environmental humidity. These chitosan nanofiber meshes were developed as a culture substrate for hepatocytes. The fibers exhibited a uniform diameter distribution (average diameter: 112 nm) and FTIR results indicate that the chemical structure of chitosan is stable during the electrospinning process. The attachment, morphology and activity of hepatocytes cultured on the chitosan nanofiber meshes were tested. The results showed that the chitosan nanofibers are biocompatible with hepatocytes and that these chitosan nanofiber meshes could be useful tissue culture substrates for various applications, including bioartificial liver-assist devices and tissue engineering for liver regeneration.
在这项研究中，我们开发了一种方法来获得由多种分子量壳聚糖组成的高表面积纳米纤维网。该方法需要降低壳聚糖溶液的粘度和表面张力，并优化静电纺丝参数，如施加电压和环境湿度。这些壳聚糖纳米纤维网被开发为肝细胞的培养底物。纤维表现出均匀的直径分布（平均直径：112nm），FTIR 结果表明壳聚糖的化学结构在静电纺丝过程中是稳定的。对培养在壳聚糖纳米纤维网上的肝细胞的附着、形态和活性进行了测试。结果表明，壳聚糖纳米纤维与肝细胞具有生物相容性，并且这些壳聚糖纳米纤维网可作为各种应用的有用组织培养底物，包括生物人工肝辅助装置和肝再生的组织工程。
