人肝癌细胞系（HepG2）对低温应激及恢复的细胞反应。热休克蛋白70（Hsp70）、热休克蛋白60（Hsp60）和热休克因子1（Hsf1）表达的诱导。
Human hepatoma cell line (HepG2) cellular response to hypothermic stress with recovery. Induction of Hsp70, Hsp60 and Hsf1 expression.
作者信息
Rada Alegna, Merentes Elizabeth, Rodríguez Marianela, Anselmi Guillermo, Strauss Mirian
机构信息
Sección de Biología Celular, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
出版信息
Invest Clin. 2010 Dec;51(4):479-88.
The cell response of human HepG2 cells exposed to hypothermia with rewarming was analyzed. Ultrastructural findings in hypothermic stressed cells showed swollen mitochondria, dispersed chromatin, vacuoles and ring-shape nucleolar reorganization. These changes were coupled with significative differences in the induction of Hsp60, inducible Hsp70 and monomeric Hsfl in all treated samples, but not in Hsc 70 expression. Cellular response to hypothermia could be associated with the synergistic induction of Hsp expression.
分析了复温的低温处理下人HepG2细胞的细胞反应。低温应激细胞的超微结构发现显示线粒体肿胀、染色质分散、液泡和环形核仁重组。所有处理样本中，这些变化与热休克蛋白60（Hsp60）、诱导型热休克蛋白70（Hsp70）和单体热休克因子1（Hsfl）诱导的显著差异相关，但与热休克蛋白70（Hsc 70）表达无关。细胞对低温的反应可能与热休克蛋白表达的协同诱导有关。
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