序列互补驱动的 RNA 非酶连接。

Sequence complementarity-driven nonenzymatic ligation of RNA.

机构信息

Dipartimento di Biologia e Biotecnologie Charles Darwin, Sapienza Università di Roma, P.le Aldo Moro, 5, Rome 00185, Italy.

出版信息

Biochemistry. 2011 Apr 12;50(14):2994-3003. doi: 10.1021/bi101981z. Epub 2011 Mar 16.

DOI:10.1021/bi101981z

PMID:21361363

Abstract

We report two reactions of RNA G:C sequences occurring nonenzymatically in water in the absence of any added cofactor or metal ion: (a) sequence complementarity-driven terminal ligation and (b) complementary sequence adaptor-driven multiple tandemization. The two abiotic reactions increase the chemical complexity of the resulting pool of RNA molecules and change the Shannon information of the initial population of sequences.

摘要

我们报告了 RNA G:C 序列在水相中非酶促反应的两种反应：（a）序列互补驱动的末端连接和（b）互补序列接头驱动的多次串联。这两种非生物反应增加了反应产物 RNA 分子的化学复杂性，并改变了初始序列群体的香农信息量。