Ji Wen-Can, Ding Kai-Yang, Zhu Wei-Bo, Wang Jian, Zhang Lei, Wu Jing-Sheng
Department of Hematology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Feb;19(1):90-3.
This study was aimed to investigate the expression of CCAAT/enhancer binding protein alpha gene (c/ebpα) in patients with myelodysplastic syndromes (MDS) and to explore the significance of c/ebpα in pathogenesis and progression of MDS. Real time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) method was used to detect the expression level of c/ebpα mRNA in bone marrow mononuclear cells (BMMNC) of 33 patients with MDS and 14 normal controls. The results showed that the expression level of c/ebpα mRNA in low-risk and high-risk MDS was significantly lower than that of normal controls (p < 0.01, p < 0.001, respectively), moreover, high-risk MDS showed lower c/ebpα mRNA expression compared with low-risk MDS (p < 0.05). c/ebpα mRNA expression level in MDS was not correlated with sex, age and peripheral blood cell amount, while the ratio of blast cells in bone marrow was in the c/ebpα mRNA low expression group significantly higher than that in the high expression group (p < 0.01). It is concluded that down-regulation of c/ebpα mRNA expression level has closely associated with the pathogenesis of MDS, the c/ebpα may be an important molecular biological marker of MDS; the degree of down-regulated c/ebpα has closely related to the progression of MDS.
本研究旨在探讨CCAAT/增强子结合蛋白α基因（c/ebpα）在骨髓增生异常综合征（MDS）患者中的表达情况，并探讨c/ebpα在MDS发病机制及病情进展中的意义。采用实时定量逆转录聚合酶链反应（RQ-PCR）方法检测33例MDS患者及14例正常对照者骨髓单个核细胞（BMMNC）中c/ebpα mRNA的表达水平。结果显示，低危和高危MDS患者c/ebpα mRNA的表达水平均显著低于正常对照者（分别为p<0.01，p<0.001），而且高危MDS患者c/ebpα mRNA的表达低于低危MDS患者（p<0.05）。MDS患者c/ebpα mRNA表达水平与性别、年龄及外周血细胞数量无关，而骨髓中原始细胞比例在c/ebpα mRNA低表达组显著高于高表达组（p<0.01）。结论：c/ebpα mRNA表达水平下调与MDS的发病机制密切相关，c/ebpα可能是MDS重要的分子生物学标志物；c/ebpα下调程度与MDS病情进展密切相关。
