Athletics Department and Graduate School, National Taiwan College of Physical Education, Taichung, Taiwan, China.
Chin Med J (Engl). 2011 Feb;124(4):530-6.
It is well known that dehydration can impair bodily functions. To evaluate the impact of hydration status under ambient environmental temperature on the immune system, 25 male collegiate wrestlers were recruited to undergo an experimental dehydration program.
Thirteen subjects had controlled diets with individual energy requirements to prevent body mass loss and restricted water intake to cause 4.52% dehydration; they formed the dehydrated group (DE). These subjects developed a urine specific gravity of about 1.030 in 84 hours. Twelve other subjects had no water restriction and maintained their total body weight comprised the euhydrated group (EU). Peripheral blood monocytes (PBMNC) were isolated after dehydration to perform immune response testing by being incubated with a polysaccharide fraction from fu-ling, Poria cocos (polysaccharide fraction from Poria cocos, PCPS, 1 - 30 £g/L), to prepare a conditioned medium termed conditioned medium of PBMNC stimulated by PCPS (PCPS-MNC-CM). More PCPS (25 µg/L) was needed in the DE group to prepare the PCPS-MNC-CM, which was assayed with a growth inhibitory curve for treated U973 cells.
The treated U937 cells, incubated together with PCPS-MNC-CM from the DE group, exhibited a much lower nitroblue tetrazolium (NBT) positive value of (63.7 ± 4.7)%. The concentration of interferon-gamma (IFN-γ), interleukin (IL)-1β and tumor necrosis factor (TNF)-α in PCPS-MNC-CM from subjects after dehydration was much lower than in the CM from the EU group.
The immune response to PCPS in the DE group was lower than in normally hydrated subjects.
众所周知，脱水会损害身体机能。为了评估环境温度下的水合状态对免疫系统的影响，招募了 25 名男性大学生摔跤手进行实验性脱水计划。
13 名受试者按照个人能量需求进行控制饮食，以防止体重减轻，并限制水分摄入，造成 4.52%的脱水；他们形成了脱水组（DE）。这些受试者在 84 小时内尿液比重达到约 1.030。另外 12 名受试者没有限制水分摄入，保持了他们的总体重，组成了水合组（EU）。在脱水后分离外周血单核细胞（PBMNC），通过与茯苓多糖（PCPS，1-30 £g/L）孵育来进行免疫反应测试，制备称为 PCPS 刺激的 PBMNC 条件培养基（PCPS-MNC-CM）。DE 组需要更多的 PCPS（25 µg/L）来制备 PCPS-MNC-CM，并用处理的 U973 细胞的生长抑制曲线进行测定。
与来自 DE 组的 PCPS-MNC-CM 一起孵育的处理 U937 细胞的硝基四唑蓝（NBT）阳性值（63.7 ± 4.7）%明显较低。来自 DE 组受试者的 PCPS-MNC-CM 中的干扰素-γ（IFN-γ）、白细胞介素（IL）-1β 和肿瘤坏死因子（TNF）-α的浓度明显低于 EU 组 CM。
DE 组对 PCPS 的免疫反应低于正常水合组。
