Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography.
作者信息
Teller J K, Fahien L A
机构信息
Department of Pharmacology, University of Wisconsin Medical School, Madison 53706.
出版信息
Protein Expr Purif. 1990 Nov;1(2):151-4. doi: 10.1016/1046-5928(90)90009-n.
PMID:2136236
Abstract
A two-step chromatographic procedure, based on a specific ligand-binding approach, for the purification of tumor NAD(P)(+)-dependent malic enzyme is described. The enzyme was purified to near homogeneity by extraction from mitochondria, negative cellulose phosphate chromatography, ammonium sulfate precipitation, and application of specific elution from a malate-agarose column. The rationale for the use of the affinity column is also described.
摘要
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