Department of Rheumatology and Immunology, Clinical Immunology Center, Beijing University People's Hospital, 11 Xizhimen South Street, Beijing, China.
J Rheumatol. 2011 May;38(5):821-7. doi: 10.3899/jrheum.100089. Epub 2011 Mar 1.
To explore the potential role of Dickkopf-1 (DKK-1) in rheumatoid arthritis (RA) and to evaluate the effect of a tumor necrosis factor-α (TNF-α) inhibitor (infliximab) and an interleukin 1 receptor antagonist (IL-1Ra; anakinra) on DKK-1 secretion in patients with RA.
Serum samples were collected from 100 patients with RA, 100 patients with other rheumatic diseases (e.g., osteoarthritis and ankylosing spondylitis), and 40 healthy controls. DKK-1 and osteoprotegerin (OPG) levels in serum were detected by ELISA. Serum C-reactive protein (CRP) levels, erythrocyte sedimentation rates (ESR), rheumatoid factor (RF) titers, and anti-cyclic citrullinated peptide antibody were also measured in patients with RA.
The serum level of DKK-1 was significantly higher in patients with RA than in healthy controls and those with other rheumatic diseases (p < 0.01); the serum DKK-1 level was correlated with levels of CRP (r = 0.488, p = 0.003) and ESR (r = 0.458, p = 2.4 x 10(-4)) and the Sharp score of radiologic change (r = 0.449, p = 0.001) in RA. In contrast to the increasing level of OPG, DKK-1 was significantly decreased in RA patients treated with TNF-α inhibitor (p < 0.01). DKK-1 was significantly decreased in RA patients treated with IL-1Ra (p < 0.01).
DKK-1, as an important mediator, was correlated with bone erosion and inflammation in RA. The change of DKK-1 level may serve as a biomarker of disease activity and bone erosion.
探讨 Dickkopf-1（DKK-1）在类风湿关节炎（RA）中的潜在作用，并评估肿瘤坏死因子-α（TNF-α）抑制剂（英夫利昔单抗）和白细胞介素 1 受体拮抗剂（IL-1Ra；阿那白滞素）对 RA 患者 DKK-1 分泌的影响。
收集 100 例 RA 患者、100 例其他风湿病（如骨关节炎和强直性脊柱炎）患者和 40 名健康对照者的血清样本。采用 ELISA 法检测血清 DKK-1 和骨保护素（OPG）水平。RA 患者还检测了血清 C 反应蛋白（CRP）水平、红细胞沉降率（ESR）、类风湿因子（RF）滴度和抗环瓜氨酸肽抗体。
RA 患者血清 DKK-1 水平明显高于健康对照组和其他风湿病患者（p < 0.01）；血清 DKK-1 水平与 CRP（r = 0.488，p = 0.003）和 ESR（r = 0.458，p = 2.4 x 10(-4)）以及 RA 患者放射学变化 Sharp 评分（r = 0.449，p = 0.001）呈正相关。与 OPG 水平升高相反，TNF-α 抑制剂治疗的 RA 患者 DKK-1 水平明显降低（p < 0.01）。IL-1Ra 治疗的 RA 患者 DKK-1 水平明显降低（p < 0.01）。
作为重要的介质，DKK-1 与 RA 中的骨侵蚀和炎症相关。DKK-1 水平的变化可能作为疾病活动和骨侵蚀的生物标志物。
