Microinjecting cells using a constant-flow microinjection system.
作者信息
Dean David A, Gasiorowski Joshua Z
出版信息
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):prot5590. doi: 10.1101/pdb.prot5590.
Abstract
摘要
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本文引用的文献
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Nonviral gene delivery.非病毒基因递送
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):top101. doi: 10.1101/pdb.top101.
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Microinjecting cells using a pulsed-flow microinjection system.
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):prot5589. doi: 10.1101/pdb.prot5589.
3
DNA sample preparation and loading sample into pipettes for microinjection of cells.
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):prot5588. doi: 10.1101/pdb.prot5588.
4
Preparing injection pipettes on a PUL-1 micropipette puller.
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Preparing injection pipettes on a flaming/brown pipette puller.
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Plating cells for microinjection.
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