Nonviral gene delivery.
作者信息
Dean David A, Gasiorowski Joshua Z
出版信息
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):top101. doi: 10.1101/pdb.top101.
Abstract
摘要
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本文引用的文献
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2
Microinjecting cells using a pulsed-flow microinjection system.
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3
DNA sample preparation and loading sample into pipettes for microinjection of cells.
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4
Preparing injection pipettes on a PUL-1 micropipette puller.
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Preparing injection pipettes on a flaming/brown pipette puller.
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Plating cells for microinjection.
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Dendrimer-mediated transfection.
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Liposome-mediated transfection.
Cold Spring Harb Protoc. 2011 Mar 1;2011(3):prot5583. doi: 10.1101/pdb.prot5583.
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What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues.关于电穿孔介导基因在细胞和组织中转移与表达的机制，（仍）有哪些未知之处。
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