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本文引用的文献

1
Is GAPDH a relevant housekeeping gene for normalisation in colorectal cancer experiments?甘油醛-3-磷酸脱氢酶(GAPDH)是结直肠癌实验中用于标准化的合适管家基因吗?
Br J Cancer. 2010 Oct 26;103(9):1475-6. doi: 10.1038/sj.bjc.6605851. Epub 2010 Sep 21.
2
Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma.定量表达分析及 L-DOPA 脱羧酶在结直肠腺癌中的预后意义。
Br J Cancer. 2010 Apr 27;102(9):1384-90. doi: 10.1038/sj.bjc.6605654.
3
Normalizing RT-qPCR data: are we getting the right answers? An appraisal of normalization approaches and internal reference genes from a case study in the finfish Lates calcarifer.标准化 RT-qPCR 数据:我们得到的答案正确吗?来自鱼类尖吻鲈 Lates calcarifer 的案例研究对标准化方法和内参基因的评估。
Mar Biotechnol (NY). 2011 Apr;13(2):170-80. doi: 10.1007/s10126-010-9277-z. Epub 2010 Mar 23.
4
Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR.采用实时定量 PCR 技术研究大鼠少突胶质细胞基因表达的内参基因选择。
J Neurosci Methods. 2010 Mar 15;187(1):78-83. doi: 10.1016/j.jneumeth.2009.12.018. Epub 2009 Dec 29.
5
One-step quantification of single-stranded DNA in the presence of RNA using Oligreen in a real-time polymerase chain reaction thermocycler.在实时聚合酶链反应热循环仪中使用Oligreen在RNA存在的情况下对单链DNA进行一步定量。
Anal Biochem. 2008 Jan 1;372(1):116-8. doi: 10.1016/j.ab.2007.08.023. Epub 2007 Aug 23.
6
Gene expression in human skeletal muscle: alternative normalization method and effect of repeated biopsies.人类骨骼肌中的基因表达:替代标准化方法及重复活检的影响。
Eur J Appl Physiol. 2005 Oct;95(4):351-60. doi: 10.1007/s00421-005-0022-7. Epub 2005 Oct 27.
7
Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise.急性运动后人骨骼肌中管家基因的实时逆转录聚合酶链反应分析
Physiol Genomics. 2004 Jul 8;18(2):226-31. doi: 10.1152/physiolgenomics.00067.2004.

Choosing a stable housekeeping gene and protein is essential in generating valid gene and protein expression results.

作者信息

Sullivan-Gunn M, Hinch E, Vaughan V, Lewandowski P

出版信息

Br J Cancer. 2011 Mar 15;104(6):1055; author reply 1056. doi: 10.1038/bjc.2011.35. Epub 2011 Mar 1.

DOI:10.1038/bjc.2011.35
PMID:21364583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3065275/
Abstract
摘要