Canales-Treviño M L, Tsutsumi V, Martínez-Palomo A
Sección de Patología Experimental, Centro de Inv. y Estudios Avanzados del Instituto Politécnico Nacional, México, D.F.
Arch Invest Med (Mex). 1990;21 Suppl 1:81-4.
The mechanisms of host resistance in hepatic amebiasis are poorly understood. Previous studies in guinea-pigs have shown that amebic liver infection is mild and cures spontaneously in approximately four days, in contrast to hamsters, where amebic liver infection progresses to coalescent abscesses that kill the animals in about one month. To determine the role of macrophages in the resistance of guinea-pigs to hepatic amebic infection, animals were treated with intraportal injection of Entamoeba histolytica, using the same route. Results showed that the amebic hepatic lesions are larger and require longer time to cure in the silica-treated group. This suggests that macrophages play a role in the resistance of the guinea-pig to amebic liver infection. The possible dependence of T lymphocytes in the resistance of guinea pigs to amebic hepatic infection was analyzed using Cyclosporin A. Results showed that resistance to E. histolytica liver infection is not related to circulating T lymphocytes. We conclude that the guinea-pig is a suitable experimental model for the study of natural host resistance mechanisms in hepatic amebiasis. Macrophages, but not T lymphocytes participate as an effective cellular immune response to eradicate amebic infection in the liver of guinea-pigs.
目前对肝脏阿米巴病宿主抵抗力的机制了解甚少。先前在豚鼠身上的研究表明，阿米巴肝感染症状较轻，大约四天后可自行痊愈，而仓鼠的情况则相反，其阿米巴肝感染会发展为融合性脓肿，约一个月内导致动物死亡。为了确定巨噬细胞在豚鼠抵抗肝脏阿米巴感染中的作用，采用相同途径通过门静脉注射溶组织内阿米巴对动物进行治疗。结果显示，在二氧化硅处理组中，阿米巴肝损伤更大，治愈所需时间更长。这表明巨噬细胞在豚鼠抵抗阿米巴肝感染中发挥作用。使用环孢素A分析了T淋巴细胞在豚鼠抵抗阿米巴肝感染中的可能依赖性。结果表明，对溶组织内阿米巴肝感染的抵抗力与循环T淋巴细胞无关。我们得出结论，豚鼠是研究肝脏阿米巴病天然宿主抵抗机制的合适实验模型。巨噬细胞而非T淋巴细胞参与了清除豚鼠肝脏中阿米巴感染的有效细胞免疫反应。
