用于高通量测序的小RNA cDNA文库构建

Construction of small RNA cDNA libraries for high-throughput sequencing.

作者信息

Lu Cheng, Shedge Vikas

机构信息

Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.

出版信息

Methods Mol Biol. 2011;729:141-52. doi: 10.1007/978-1-61779-065-2_9.

DOI:10.1007/978-1-61779-065-2_9

PMID:21365488

Abstract

Small RNAs (smRNAs) play an essential role in virtually every aspect of growth and development, by regulating gene expression at the post-transcriptional and/or transcriptional level. New high-throughput sequencing technology allows for a comprehensive coverage of smRNAs in any given biological sample, and has been widely used for profiling smRNA populations in various developmental stages, tissue and cell types, or normal and disease states. In this article, we describe the method used in our laboratory to construct smRNA cDNA libraries for high-throughput sequencing.

摘要

小RNA（smRNA）通过在转录后和/或转录水平上调控基因表达，在生长和发育的几乎每个方面都发挥着重要作用。新的高通量测序技术能够全面覆盖任何给定生物样品中的smRNA，并已广泛用于分析不同发育阶段、组织和细胞类型或正常与疾病状态下的smRNA群体。在本文中，我们描述了我们实验室用于构建用于高通量测序的smRNA cDNA文库的方法。