INS-1E 细胞的二维凝胶蛋白质组参考图谱。

Two-dimensional gel proteome reference map of INS-1E cells.

机构信息

Laboratory for Experimental Medicine and Endocrinology, Catholic University of Leuven, Leuven, Belgium.

出版信息

Proteomics. 2011 Apr;11(7):1365-9. doi: 10.1002/pmic.201000006. Epub 2011 Feb 17.

DOI:10.1002/pmic.201000006

Abstract

The insulin-producing INS-1E rat cell line is widely used as a model for studying β-cells. It is a well-characterized cell line, mainly used in diabetes research. We established a 2-DE reference map for INS-1E cells. Using MALDI-TOF/TOF-MS/MS, we identified 546 spots. These included various proteins with an important role in β-cell physiology and with known roles as crucial proteins for diabetes development. We believe that the availability of this reference map will enhance our knowledge of β-cell physiology.

摘要

胰岛素分泌细胞系 INS-1E 是一种广泛用于研究β细胞的模式细胞系。它是一种特征明确的细胞系，主要用于糖尿病研究。我们建立了 INS-1E 细胞的 2-DE 参考图谱。使用 MALDI-TOF/TOF-MS/MS，我们鉴定了 546 个点。这些蛋白在β细胞生理学中具有重要作用，并且已知是糖尿病发展的关键蛋白。我们相信，这个参考图谱的出现将增强我们对β细胞生理学的认识。