Sales-product Department, Anatech Corporation, Tokyo, Japan.
Proteomics. 2011 Apr;11(8):1545-9. doi: 10.1002/pmic.201000314. Epub 2011 Feb 25.
We assessed whether the G-electrode-loading method (GELM) was helpful in the protein analysis. GELM in 2-DE was compared with the slip-loading, the in-gel rehydration and the cup-loading in 2-DE. GELM showed the best results for protein separation. A total of 14 spots that showed an increase with GELM were analyzed by MALDI-TOF MS. In GELM, all of these spots were identified with a high score and a high sequence coverage. A membrane-associated protein was identified and determined to have phosphorylated site. These tests show that GELM has several advantages for protein analysis compared with the traditional methods.
我们评估了 G 电极加载方法（GELM）在蛋白质分析中的作用。将 GELM 与 2-DE 中的滑加载、胶内再水化和杯加载进行了比较。GELM 在蛋白质分离方面表现最佳。通过 MALDI-TOF MS 对显示出与 GELM 增加相关的 14 个斑点进行了分析。在 GELM 中，所有这些斑点都被鉴定出具有高得分和高序列覆盖率。鉴定出一种膜相关蛋白，并确定其具有磷酸化位点。这些测试表明，与传统方法相比，GELM 在蛋白质分析方面具有若干优势。
