Röhm-Rodowald Ewa, Jakimiak Bozenna, Podgórska Marta, Chojecka Agnieszka
Zakład Zwalczania Skazeń Biologicznych, Narodowy Instytut Zdrowia Publicznego-Państwowy Zakład Higieny, Warszawa.
Rocz Panstw Zakl Hig. 2010;61(3):329-33.
Thermal disinfection should be applied to laundering procedures of hospital textiles contaminated with blood. Currently, there is an increasing number of hospital textiles composed of cotton-polyester blends that cannot endure high temperatures of thermal disinfection. Besides, decreasing the temperature of chemothermal disinfection enhances the possibility of micro-organisms to survive the laundering procedure. The aim of this study was to prepare a new method for the microbiological evaluation of disinfecting laundering procedures for hospital textiles contaminated with blood. The bactericidal activity of chemical disinfectants for chemothermal disinfection was determined by simulating a laundering procedure for hospital textiles in the laboratory according to procedure of National Institute of Hygiene - DF/05/03. Bioindicators cotton carriers inoculated with Enterococcus faecium were used for determinating the antibacterial effects for hospital textiles contaminated with blood. High concentrations of bovine albumin and/or sheep erythrocytes were used as substrate for simulating human blood. The results showed that the bactericidal activity of chemical disinfectants for chemothermal disinfection hospital textiles in the event of massive organic contamination--heavily soiled with blood, shall be evaluated using carrier test in following conditions: test organism- Enterococcus faecium, interfering substances--6 g/l bovine albumin solution added to preparation.
热消毒应应用于被血液污染的医院织物的洗涤程序。目前,由棉涤混纺制成的医院织物数量不断增加,这些织物无法承受热消毒所需的高温。此外,降低化学热消毒的温度会增加微生物在洗涤过程中存活的可能性。本研究的目的是制备一种新的方法,用于对被血液污染的医院织物消毒洗涤程序进行微生物学评估。根据国家卫生研究所的程序-DF/05/03,在实验室中模拟医院织物的洗涤程序,测定用于化学热消毒的化学消毒剂的杀菌活性。使用接种了粪肠球菌的生物指示剂棉载体来测定对被血液污染的医院织物的抗菌效果。高浓度的牛白蛋白和/或羊红细胞用作模拟人血的底物。结果表明,在大量有机污染(严重被血液污染)的情况下,用于化学热消毒医院织物的化学消毒剂的杀菌活性应在以下条件下使用载体试验进行评估:试验微生物-粪肠球菌,干扰物质-向制剂中添加6 g/l牛白蛋白溶液。