Hannaoui Erika, Villalobos Luz, Martínez Rosa, Maldonado Antonio, Hagel Isabel, Bastardo Jesús
Universidad de Oriente, Núcleo de Sucre, Cumaná, Venezuela.
Invest Clin. 2010 Dec;51(4):489-500.
To establish the prevalence of strains of diarrheagenic Escherichia coli (DEC) associated to acute diarrhea in children of Cumaná, Venezuela, stool samples were taken from 200 children aged < 5 years with acute diarrheal disease, and from 30 healthy children used as control. Isolation and bacterial identification was performed by conventional biochemical tests and stool cultures. The presence of pathogenic genes of each type of DEC was investigated by the technique of polymerase chain reaction (PCR), determining the genes eae and bfp (EPEC), st and lt (ETEC), ipaH and virF (EIEC) Stx1/Stx2 (STEC), aafII (EAEC) and daaE (ADEC). From 169 E. coli, isolates we determined by PCR 10.65% positive for EPEC (1.18% "typical", 9.47% "atypical"); ETEC (5.91%); EAEC (1.78 %), EIEC (0.59%). There were no statistically significant differences regarding the frequency of each "pathotype" in relation to age, but it did occur when related to the sex (p<0.05). The most relevant clinical features were: fever, vomiting and abdominal pain and the greatest percentage of children affected were of the working and marginal classes. These results shown that the strains of DEC are important etiological agents in acute infectious diarrhea in children of Cumaná.
为确定与委内瑞拉库马纳市儿童急性腹泻相关的致泻性大肠杆菌（DEC）菌株的流行情况，从200名年龄小于5岁患有急性腹泻病的儿童以及30名作为对照的健康儿童中采集粪便样本。通过常规生化试验和粪便培养进行分离和细菌鉴定。采用聚合酶链反应（PCR）技术研究每种DEC类型的致病基因，检测eae和bfp基因（肠致病性大肠杆菌，EPEC）、st和lt基因（肠产毒性大肠杆菌，ETEC）、ipaH和virF基因（肠侵袭性大肠杆菌，EIEC）、Stx1/Stx2基因（肠出血性大肠杆菌，STEC）、aafII基因（肠集聚性大肠杆菌，EAEC）和daaE基因（粘附性大肠杆菌，ADEC）。在169株大肠杆菌分离株中，通过PCR确定EPEC阳性率为10.65%（“典型”的为1.18%，“非典型”的为9.47%）；ETEC为5.91%；EAEC为1.78%；EIEC为0.59%。各“致病型”的频率在年龄方面无统计学显著差异，但在性别方面存在差异（p<0.05）。最相关的临床特征为：发热、呕吐和腹痛，受影响儿童比例最高的是工人阶级和边缘阶层。这些结果表明，DEC菌株是库马纳市儿童急性感染性腹泻的重要病原体。
