Animal Medicine and Surgery Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, Ciudad Universitaria, 28040 Madrid, Spain.
Anim Reprod Sci. 2011 Mar;124(1-2):85-9. doi: 10.1016/j.anireprosci.2011.02.001. Epub 2011 Feb 16.
For many years in human assisted-reproduction procedures there have been special protocols to prepare and improve sperm quality. Colloidal centrifugation (CC) is a useful technique that has been proved to enhance semen quality by selection of the best spermatozoa for different species. Its use is recommended to improve fertility of subfertile stallions but current CC protocols are clinically complicated in the equine sperm processing technique due to economic and technical difficulties. The aim of this study was to determine the optimal processing procedures to adapt the use of a CC product (EquiPure™) in the equine reproduction industry. A total of nineteen ejaculates were collected from 10 Purebred Spanish Horses (P.R.E horses) using a Missouri artificial vagina. Gel-free semen aliquots were analyzed prior to treatment (control). Semen was subjected to one of six CC protocols with EquiPure™ and centrifuged samples were statistically evaluated by ANOVA and Duncan tests (p<0.05) for sperm quality and recovery rate. We obtained higher values by colloidal centrifugation in LIN, STR and BCF variables and DNA fragmentation index trended to be lower in most of the CC protocols. The studied protocols were shown to be as efficient in improving equine sperm quality as the current commercial EquiPure™, with the added advantage of being much more economical and simple to use. According to these results it seems to be possible to incorporate single layer and or high colloidal centrifugation volume protocols what would make them simple, economic and clinically viable for the equine sperm processing procedure.
多年来，在人类辅助生殖程序中，一直有专门的方案来准备和改善精子质量。胶粒离心（CC）是一种有用的技术，已经被证明可以通过选择最佳的精子来提高不同物种的精液质量。它的使用被推荐用于提高亚生育种马的生育能力，但由于经济和技术上的困难，目前 CC 方案在马的精子处理技术中临床操作复杂。本研究的目的是确定最佳的处理程序，以适应 CC 产品（EquiPure™）在马繁殖产业中的使用。使用密苏里人工阴道从 10 匹西班牙纯种马（P.R.E 马）中采集了 19 份精液。在处理前（对照）分析无凝胶精液等分试样。精液经过 EquiPure™的六种 CC 方案之一处理，离心样品通过 ANOVA 和 Duncan 检验进行统计学评估（p<0.05），以评估精子质量和回收率。我们通过胶体离心在 LIN、STR 和 BCF 变量中获得了更高的值，并且在大多数 CC 方案中 DNA 碎片化指数呈下降趋势。研究的方案在改善马精子质量方面与当前的商业 EquiPure™一样有效，而且具有更经济、更简单的使用优势。根据这些结果，似乎可以采用单层和/或高胶体离心体积方案，这将使它们在马的精子处理程序中简单、经济和临床可行。
