Federal University of Mato Grosso do Sul, Faculty of Veterinary Medicine and Animal Science, Av. Filinto Muller, 2443. Cidade Universitária, Campo Grande, MS 79070-900, Brazil.
Anim Reprod Sci. 2011 Mar;124(1-2):98-103. doi: 10.1016/j.anireprosci.2011.01.017. Epub 2011 Feb 1.
This study aimed to characterize the stages of the seminiferous epithelium cycle by the tubular morphology method, and to determine the number of differentiated spermatogonia generations in the adult white-lipped peccary. Twenty adult white-lipped peccaries, obtained from commercial slaughterhouse, were used. Fragments of the testicular parenchyma were fixed in 3% glutaraldehyde and embedded into a methacrylate resin. The number of germ and Sertoli cells was estimated by the analysis of cell populations in 50 transversal sections of seminiferous tubules in different stages of the cycle. The tubular morphology method allowed the identification of cellular associations characteristic of the eight stages of the seminiferous epithelium cycle in white-lipped peccaries. The results showed the presence of six generations of differentiated spermatogonia in white-lipped peccaries, and that the cell composition of the eight stages of the seminiferous epithelium cycle in this species is very similar to that described for collared peccaries.
本研究旨在通过管状形态学法来描述精小管上皮周期的各个阶段，并确定成年白唇野猪中分化精原细胞的世代数。本研究使用了 20 头来自商业屠宰场的成年白唇野猪。将睾丸实质的片段固定在 3%戊二醛中，并嵌入甲基丙烯酸树脂中。通过对处于不同周期阶段的 50 个精小管横切片中的细胞群体进行分析，估计精细胞和支持细胞的数量。管状形态学法可识别出白唇野猪精小管上皮周期的八个阶段的特征性细胞组合。结果表明，白唇野猪中存在六代分化精原细胞，并且该物种的精小管上皮周期的八个阶段的细胞组成与颈带野猪的描述非常相似。
