National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
Biotechnol Lett. 2011 Jul;33(7):1457-63. doi: 10.1007/s10529-011-0572-1. Epub 2011 Mar 3.
Spider venoms are neurotoxin proteins that can kill insects. Spider toxin Hvt gene was cloned under two phloem specific RSs1 and RolC promoters, transformed into tobacco plants through Agrobacterium-mediated transformation and tested against Heliothis armigera larvae. Transgenic plants were confirmed through PCR. First instar larvae of H. armigera were released on detached leaves of transformed and non-transformed plants. Insect bioassays showed 93-100% mortality of H. armigera larvae within 72 h on the leaves of transgenic plants while all larvae survived and continued feeding on detached leaves from non-transformed control plants. The Hvt gene expressing under phloem specific RSs1 and RolC promoters could therefore be used for developing H. armigera-resistant, genetically-modified crops.
蜘蛛毒液是能够杀死昆虫的神经毒素蛋白。本研究利用两个韧皮部特异性启动子 RSs1 和 RolC 克隆蜘蛛毒素 Hvt 基因，通过根癌农杆菌介导转化法将其转化到烟草植株中，并对其进行了对棉铃虫幼虫的测试。通过 PCR 对转基因植株进行了确认。将棉铃虫的一龄幼虫释放到转化和非转化植株的离体叶片上。昆虫生物测定显示，在转基因植株叶片上，93%-100%的棉铃虫幼虫在 72 小时内死亡，而所有幼虫在非转化对照植株的离体叶片上存活并继续取食。因此，在韧皮部特异性启动子 RSs1 和 RolC 表达的 Hvt 基因可用于开发抗棉铃虫的转基因作物。
