Brandt F H, Eberhard M L
Division of Parasitic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Parasitol. 1990 Feb;76(1):93-5.
Three routes of inoculation were compared to determine the best method to infect ferrets with Dracunculus insignis. The traditional method of administering infected cyclops containing L3s through gavage was compared to intraperitoneal (i.p.) and subcutaneous (s.c.) inoculation of L3s. Ten of 18 (56%) gavaged ferrets became infected after receiving copepods containing approximately 100 L3s each; 44 adult worms were recovered from these 10 animals. Twenty-one of 28 (75%) animals inoculated with 50 L3s each became infected i.p.; 92 adult worms were recovered from the positive animals. Four of 5 (80%) ferrets given subcutaneous inoculations of 50 L3s became infected; only 6 worms were recovered from these 4 animals. Inoculation of larvae via the i.p. or s.c. route greatly simplifies the infection procedure and produces more consistent results. A simple procedure is described, which permits rapid recovery of L3s to be used in the i.p. or s.c. inoculations.
比较了三种接种途径，以确定用 insignis 龙线虫感染雪貂的最佳方法。将通过灌胃给予含有 L3 期幼虫的感染剑水蚤的传统方法与 L3 期幼虫的腹腔内（i.p.）和皮下（s.c.）接种进行了比较。18 只接受灌胃的雪貂中有 10 只（56%）在接受每只含有约 100 条 L3 期幼虫的剑水蚤后被感染；从这 10 只动物中回收了 44 条成虫。28 只每只接种 50 条 L3 期幼虫的动物中有 21 只（75%）通过腹腔内接种被感染；从阳性动物中回收了 92 条成虫。5 只接受皮下接种 50 条 L3 期幼虫的雪貂中有 4 只（80%）被感染；从这 4 只动物中仅回收了 6 条虫。通过腹腔内或皮下途径接种幼虫极大地简化了感染程序，并产生了更一致的结果。描述了一种简单的程序，该程序允许快速回收用于腹腔内或皮下接种的 L3 期幼虫。
