Boncler Magdalena, Rywaniak Joanna, Sicinska Paulina, Watala Cezary
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, University Hospital No. 2, 113 Zeromskiego Street, Lodz, Poland.
Blood Coagul Fibrinolysis. 2011 Jun;22(4):301-9. doi: 10.1097/MBC.0b013e3283451308.
In relation to the interplay between C-reactive protein (CRP) and its various ligands, including extracellular matrix proteins or plasma proteins, herein we compared the effectiveness of modified CRP (mCRP) in modulation of platelet function under different experimental conditions. mCRP (100 μg/ml) was significantly more effective in stimulation of platelet activation when measured in suspensions of isolated platelets than in whole blood (fraction of CD62-positive platelets was 73.4 ± 5.3 vs. 0.8 ± 1.0% in isolated platelets and 7.0 ± 1.8 vs. 1.3 ± 0.4% in whole blood). Platelet adhesion to fibrinogen was almost seven-fold higher in suspensions of isolated platelets compared to platelet-rich plasma (PRP) (P < 0.005). Furthermore, mCRP enhanced platelet aggregation in a whole blood but it had no effect in PRP. The effectiveness of mCRP in stimulation of platelet response in plasma has been associated with the proportions of gamma globulin and albumin in human serum (rp = 0.78, P < 0.0001 for gamma globulin and Rp = -0.52, P = 0.02 for albumin concentrations; for albumin/gamma globulin ratio rp = -0.72, P < 0.0005). Such associations have been further confirmed by experiments showing that mCRP interacts with some immunoglobulins. Taken together, the modulation of platelet function by mCRP seems to be strongly determined by the presence of the gamma globulin fraction in platelet milieu.
关于C反应蛋白（CRP）与其各种配体（包括细胞外基质蛋白或血浆蛋白）之间的相互作用，在此我们比较了修饰型CRP（mCRP）在不同实验条件下调节血小板功能的有效性。当在分离的血小板悬液中测量时，mCRP（100μg/ml）在刺激血小板活化方面比在全血中显著更有效（分离的血小板中CD62阳性血小板的比例为73.4±5.3%，而全血中为0.8±1.0%；全血中为7.0±1.8%，而富血小板血浆（PRP）中为1.3±0.4%）。与富血小板血浆（PRP）相比，分离的血小板悬液中血小板对纤维蛋白原的粘附几乎高7倍（P<0.005）。此外，mCRP增强全血中的血小板聚集，但对PRP无作用。mCRP在血浆中刺激血小板反应的有效性与人类血清中γ球蛋白和白蛋白的比例有关（γ球蛋白的rp=0.78，P<0.0001；白蛋白浓度的Rp=-0.52，P=0.02；白蛋白/γ球蛋白比值的rp=-0.72，P<0.0005）。通过显示mCRP与一些免疫球蛋白相互作用的实验进一步证实了这种关联。综上所述，mCRP对血小板功能的调节似乎强烈取决于血小板环境中γ球蛋白组分的存在。
