Faraci F M, Mayhan W G, Heistad D D
Department of Internal Medicine, Veterans Administration Medical Center, Iowa City, Iowa.
Am J Physiol. 1990 Jan;258(1 Pt 2):R94-8. doi: 10.1152/ajpregu.1990.258.1.R94.
The goal of this study was to examine the role of arginine vasopressin in humoral regulation of choroid plexus function. Production of cerebrospinal fluid (CSF) was measured in anesthetized rabbits with an indicator dilution method, by using ventriculocisternal perfusion of artificial CSF containing blue dextran. Rabbits received either vehicle, vasopressin or vasopressin in the presence of the V1-antagonist [1-(beta-mercapto-beta,beta-cyclopentamethylene propionic acid), 2-(O-methyl)tyrosine]arginine vasopressin ([d(CH2)5Tyr(Me)]-AVP). Under control conditions, blood flow to the choroid plexus (measured with microspheres) averaged 369 +/- 26 (mean +/- SE) ml.min-1.100 g-1 and CSF production averaged 9.9 +/- 0.9 microliters/min. Intravenous infusion of vasopressin (2 mU.kg-1.min-1 for 90 min) decreased blood flow to the choroid plexus by 50-60% for the entire period of infusion. Vasopressin decreased production of CSF by 35 +/- 8%. Blood flow to the choroid plexus and production of CSF did not change significantly from control values in animals that received vehicle. In the presence of the V1-antagonist (10 micrograms/kg), infusion of vasopressin had no effect on blood flow to the choroid plexus or production of CSF. Thus circulating vasopressin, at plasma levels that are observed under physiological and pathophysiological conditions, has important effects on formation of CSF, as well as on blood flow to the choroid plexus. These findings are consistent with the hypothesis that effects of vasopressin on both variables are mediated through vasopressin (V1)-receptors.
本研究的目的是探讨精氨酸加压素在脉络丛功能体液调节中的作用。采用指示剂稀释法，通过向脑室-脑池灌注含蓝色葡聚糖的人工脑脊液，测量麻醉兔的脑脊液生成量。兔分别接受溶剂、加压素或在V1拮抗剂[1-(β-巯基-β,β-环戊亚甲基丙酸)，2-(O-甲基)酪氨酸]精氨酸加压素([d(CH2)5Tyr(Me)]-AVP)存在下的加压素。在对照条件下，脉络丛血流量（用微球测量）平均为369±26（平均值±标准误）ml·min-1·100 g-1，脑脊液生成量平均为9.9±0.9微升/分钟。静脉输注加压素（2 mU·kg-1·min-1，持续90分钟）在整个输注期间使脉络丛血流量减少50%-60%。加压素使脑脊液生成量减少35±8%。接受溶剂的动物，其脉络丛血流量和脑脊液生成量与对照值相比无显著变化。在V1拮抗剂（10微克/千克）存在的情况下，输注加压素对脉络丛血流量或脑脊液生成量无影响。因此，在生理和病理生理条件下观察到的血浆水平的循环加压素，对脑脊液的形成以及脉络丛血流量具有重要影响。这些发现与加压素对这两个变量的作用均通过加压素（V1）受体介导的假说一致。
