Geisinger K R, Berens M E, Duckett Y, Morgan T M, Kute T E, Welander C E
Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.
Cancer. 1990 Mar 1;65(5):1055-61. doi: 10.1002/1097-0142(19900301)65:5<1055::aid-cncr2820650502>3.0.co;2-9.
The effects of estradiol (E), medroxyprogesterone acetate (MPA), and tamoxifen (TAM) on the growth of a human ovarian carcinoma cell line, BG-1, were evaluated using a tumor clonogenic assay (HTCA). BG-1 contains significant quantities of estrogen and progesterone receptors. Growth inhibition by TAM and growth stimulation by MPA were demonstrated using continuous drug exposure. Estradiol resulted in a marginal increase in colony formation. With each of these three drugs, the greatest response occurred in the larger colonies (generally greater than or equal to 60 microns). Combinations of each of these three steroidal agents with three different cytotoxic drugs were studied in the HTCA. Synergistic activities were produced with TAM combined with either cisplatin or doxorubicin. Additive effects were seen with TAM and cyclophosphamide. Although predominantly additive or synergistic, the effects were variable with MPA and all three cytotoxic agents. Combinations of estradiol with cytotoxic agents were no more active than the cytotoxics alone. These findings indicate a biological rationale for hormonal manipulation as therapy in ovarian cancer.
使用肿瘤克隆形成试验（HTCA）评估了雌二醇（E）、醋酸甲羟孕酮（MPA）和他莫昔芬（TAM）对人卵巢癌细胞系BG-1生长的影响。BG-1含有大量的雌激素和孕激素受体。通过持续药物暴露证明了TAM的生长抑制作用和MPA的生长刺激作用。雌二醇导致集落形成略有增加。使用这三种药物中的每一种，最大反应都出现在较大的集落中（通常大于或等于60微米）。在HTCA中研究了这三种甾体药物中的每一种与三种不同细胞毒性药物的组合。TAM与顺铂或多柔比星联合产生协同活性。TAM与环磷酰胺联合产生相加作用。虽然主要是相加或协同作用，但MPA与所有三种细胞毒性药物的作用是可变的。雌二醇与细胞毒性药物的组合并不比单独使用细胞毒性药物更具活性。这些发现为激素操纵作为卵巢癌治疗方法提供了生物学依据。
