绿茶多酚可预防慢性炎症诱导的雌性大鼠心肌纤维化。
Green tea polyphenols avert chronic inflammation-induced myocardial fibrosis of female rats.
机构信息
Department of Pathology, Texas Tech University Health Sciences Center, BB 198, 3601 4th street, Lubbock, TX 79430-9097, USA.
出版信息
Inflamm Res. 2011 Jul;60(7):665-72. doi: 10.1007/s00011-011-0320-y. Epub 2011 Mar 4.
OBJECTIVE
Green tea proposes anti-inflammatory properties which may attenuate chronic inflammation-induced fibrosis of vessels. This study evaluated whether green tea polyphenols (GTP) can avert fibrosis or vascular disruption along with mechanisms in rats with chronic inflammation.
TREATMENTS
Forty 3-month-old female rats were assigned to a 2 (placebo vs. lipopolysaccharide, administration) × 2 (no GTP vs. 0.5% GTP in drinking water) factorial design for 12 weeks.
METHODS
Masson's trichrome staining evaluated myocardial fibrosis in coronary vessels and surrounding myocardium. Whole blood specimens were counted for differentials. Spleen tumor necrosis factor-α (TNF-α) mRNA expression was determined by real-time RT-PCR. Data were analyzed by two-way analysis of variance (ANOVA) followed by mean separation procedures.
RESULTS
After 12 weeks, lipopolysaccharide administration induced myocardial fibrosis in vessels and surrounding myocardium, spleen TNF-α mRNA expression, and leukocytes, while GTP supplementation in drinking water significantly averted such observation.
CONCLUSIONS
GTP attenuates myocardial fibrosis through a suppression of chronic inflammation and innate immune responses.
目的
绿茶具有抗炎特性，可能减轻慢性炎症引起的血管纤维化。本研究评估了绿茶多酚（GTP）是否可以预防慢性炎症大鼠的纤维化或血管破坏及其机制。
治疗
40 只 3 月龄雌性大鼠按 2（安慰剂与脂多糖，给药）×2（无 GTP 与饮用水中 0.5%GTP）析因设计处理 12 周。
方法
Masson 三色染色评估冠状动脉血管和周围心肌的心肌纤维化。全血标本用于分类计数。实时 RT-PCR 测定脾肿瘤坏死因子-α（TNF-α）mRNA 表达。数据采用双因素方差分析（ANOVA），并进行均值分离。
结果
12 周后，脂多糖给药导致血管和周围心肌纤维化、脾 TNF-α mRNA 表达和白细胞增加，而饮用水中 GTP 补充显著预防了这种观察结果。
结论
GTP 通过抑制慢性炎症和固有免疫反应来减轻心肌纤维化。
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