College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China.
Environ Toxicol. 2012 Oct;27(10):563-72. doi: 10.1002/tox.20678. Epub 2011 Mar 3.
The effects of cypermethrin on physiological responses of three typical marine microalgal species Skeletonema costatum (Bacillariophyceae), Scrippsiella trochoidea (Dinophyceae), and Chattonella marina (Raphidophyceae), were investigated by 96-h growth tests in a batch-culture system. The 96-h median inhibition concentrations (IC(50)) were 71.4, 205, and 191 μg L(-1) for S. costatum, S. trochoidea, and C. marina, respectively. Quick and significant physiological responses occurred when algal cells were exposed to cypermethrin, and all biochemical parameters varied significantly within 6- or 12-h exposure. Cypermethrin affected algal growth, protein content, and superoxide dismutase (SOD) activity by stimulation at low concentrations (1, 5 μg L(-1)) and inhibition at high concentrations (>50 μg L(-1)). A general increase in malondialdehyde (MDA) level was observed in all test groups, which suggested that the toxic effects of cypermethrin were probably exerted through free radical generation. These results suggest that the activation of SOD and promotion of protein at early exposure are important to counteract the oxidative stress induced by cypermethrin, and the inactivation of SOD may be crucial to the growth inhibition of microalgae by cypermethrin.
采用批量培养法的 96 小时生长试验研究了氯氰菊酯对 3 种典型海洋微藻（骨条藻、旋链角毛藻和海洋卡盾藻）生理响应的影响。氯氰菊酯对骨条藻、旋链角毛藻和海洋卡盾藻的 96 小时半抑制浓度（IC（50））分别为 71.4、205 和 191μg/L。当藻类细胞暴露于氯氰菊酯时，会迅速产生显著的生理响应，所有生化参数在 6 或 12 小时暴露时均发生显著变化。氯氰菊酯在低浓度（1、5μg/L）时刺激、高浓度（＞50μg/L）时抑制藻类生长、蛋白质含量和超氧化物歧化酶（SOD）活性。所有试验组丙二醛（MDA）水平均普遍升高，表明氯氰菊酯的毒性作用可能是通过自由基生成发挥的。这些结果表明，SOD 的激活和蛋白质的早期促进对于抵御氯氰菊酯诱导的氧化应激非常重要，而 SOD 的失活可能对氯氰菊酯抑制微藻生长至关重要。
