School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
J Sep Sci. 2011 Apr;34(7):796-9. doi: 10.1002/jssc.201000856. Epub 2011 Mar 4.
The retention behavior of inorganic anions on a triazole-based stationary phase was first examined in ion chromatography. It was initially designed for hydrophilic interaction liquid chromatography and was simply prepared by introducing the triazole groups onto the surface of silica gel via click chemistry. Effective separation of common inorganic anions, including iodate, chloride, bromide, nitrate and iodide, was achieved with Na(2)SO(4) eluent. The logarithm of the retention factor of analytes was observed to be linear with the logarithm of the eluent concentration, and the slopes of the plots were almost the same as those of the ideal theoretical value. The eluent pH value in the range of 3.4-7.0 had little effect on the separation. The utility of the column was demonstrated for the determination of UV-absorbing anions in saliva and tap water.
首先在离子色谱中考察了三唑基固定相上无机阴离子的保留行为。它最初是为亲水相互作用液相色谱设计的，只需通过点击化学将三唑基团引入硅胶表面即可简单制备。使用 Na(2)SO(4)洗脱剂实现了常见无机阴离子（包括碘酸盐、氯、溴、硝酸盐和碘化物）的有效分离。分析物的保留因子的对数与洗脱剂浓度的对数呈线性关系，并且这些图的斜率几乎与理想理论值的斜率相同。洗脱剂 pH 值在 3.4-7.0 范围内对分离几乎没有影响。该柱的实用性已在唾液和自来水中测定紫外吸收阴离子中得到验证。
