School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Analyst. 2012 Apr 7;137(7):1624-8. doi: 10.1039/c2an16277f. Epub 2012 Feb 17.
A silica based amino stationary phase was prepared by immobilization of propargylamine on azide-silica via click chemistry. This readily prepared click amino stationary phase demonstrated good selectivity in separation of common inorganic anions under ion chromatography (IC) mode, and the triazole ring in combination with free amino group was observed to play a major role for separation of the anions examined. On the other hand, the stationary phase also showed good hydrophilic interaction liquid chromatography (HILIC) properties in the separation of polar compounds including nucleosides, organic acids and bases. The retention mechanism was found to match well the typical HILIC retention.
一种基于硅胶的氨基固定相通过点击化学将丙炔胺固定在叠氮硅上制备得到。这种易于制备的点击氨基固定相在离子色谱（IC）模式下对常见无机阴离子的分离表现出良好的选择性，并且观察到三唑环与游离氨基一起对所研究的阴离子的分离起主要作用。另一方面，该固定相在分离包括核苷、有机酸和碱基在内的极性化合物的亲水相互作用液相色谱（HILIC）中也表现出良好的性能。发现保留机制与典型的 HILIC 保留非常吻合。
