腺病毒介导的nm23-H1基因递送抑制结肠癌细胞系Lovo的生长

[Adenovirus-mediated delivery of nm23-H1 gene inhibits growth of colorectal carcinoma cell line Lovo].

作者信息

Wang Qi, He Xueling, Liu Yan, Yin Hailin

机构信息

School of Life Science, Sichuan University, Chengdu 610064, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Dec;27(6):1332-5.

PMID:21374989

Abstract

This experimental study sought to find out the inhibitory effects of Ad-GFP-nm23-H1 on proliferation and metastasis of human colorectal carcinoma cell line Lovo, and, further, to gain an insight into some theoretical and methodical basis for instituting nm23-H1 gene therapy of cancers. MTT assay and Transwell chamber were used to detect the rates of proliferation and invasion as well as the adhesion of Lovo cells in vitro. The results demonstrated that the proliferation inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 84.9% +/- 1.51%, 48.5% +/- 7.23% and 22.5% +/- 5.47%, that the adherence inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 70.3% +/- 2.40%, 60.1% +/- 5.68% and 18.5% +/- 3.61%, and that the invasiveness inhibition rates of Lovo cells treated with Ad-GFP-nm23-H1 of 10(10) PFU/ml, 10(9) PFU/ml and 10(8) PFU/ml were 83.2% +/- 5.71%, 52.2% +/- 6.94% and 28.1% +/- 8.21%. These data suggested that Ad-GFP-nm23-H1 exerted significant inhibitory effects on the proliferation and metastasis of human colorectal carcinoma cell line Lovo in a dose-dependent way.

摘要

本实验研究旨在探讨腺病毒介导的绿色荧光蛋白标记的nm23-H1基因（Ad-GFP-nm23-H1）对人结直肠癌细胞系Lovo增殖和转移的抑制作用，进而为开展nm23-H1基因癌症治疗提供一定的理论和方法依据。采用MTT法和Transwell小室检测Lovo细胞的体外增殖、侵袭及黏附能力。结果显示，分别用10(10) PFU/ml、10(9) PFU/ml和10(8) PFU/ml的Ad-GFP-nm23-H1处理Lovo细胞后，其增殖抑制率分别为84.9%±1.51%、48.5%±7.23%和22.5%±5.47%；黏附抑制率分别为70.3%±2.40%、60.1%±5.68%和18.5%±3.61%；侵袭抑制率分别为83.2%±5.71%、52.2%±6.94%和28.1%±8.21%。这些数据表明，Ad-GFP-nm23-H1对人结直肠癌细胞系Lovo的增殖和转移具有显著的剂量依赖性抑制作用。