[跳跃探针扫描离子电导显微镜及其在活神经元细胞拓扑成像中的应用]

[Hopping probe scanning ion conductance microscopy and its applications to topological imaging of live neuronal cells].

作者信息

Liu Xiao, Yang Xi, Zhang Xiaofan, Lu Hujie, Liu Liping, Zhang Yanjun

机构信息

China National Academy of Nanotechnology & Engineering, Tianjin 300457, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Dec;27(6):1365-9, 1378.

PMID:21374996

Abstract

As a new kind of non-contacting high-resolution scanning probe microscopy, hopping probe scanning ion conductance microscopy (HPSICM) overcomes the disadvantages of the continuous feedback-control scanning mode of the conventional scanning ion conductance microscopy (SICM), which has been restricted to imaging relatively flat biological samples. The basic operation principles of HPSICM were briefly introduced in this paper. Then the high-resolution 3D morphological images of the two live neuronal cell lines, SK-N-SH cells and NGF-differentiated neurite PC12 cells, were respectively acquired in their physiological culture environment using HPSICM. It is demonstrated that HPSICM provides a powerful microscopy for in-depth studying the microstructures and their dynamic changes of live neuronal cells in real time.

摘要

作为一种新型的非接触式高分辨率扫描探针显微镜，跳变探针扫描离子电导显微镜（HPSICM）克服了传统扫描离子电导显微镜（SICM）连续反馈控制扫描模式的缺点，传统扫描离子电导显微镜一直局限于对相对平坦的生物样品进行成像。本文简要介绍了HPSICM的基本操作原理。然后，使用HPSICM在两种活神经元细胞系（SK-N-SH细胞和NGF分化的神经突PC12细胞）的生理培养环境中分别获取了高分辨率三维形态图像。结果表明，HPSICM为实时深入研究活神经元细胞的微观结构及其动态变化提供了一种强大的显微镜技术。