使用跳跃探针离子电导显微镜进行纳米级活细胞成像。
Nanoscale live-cell imaging using hopping probe ion conductance microscopy.
作者信息
Novak Pavel, Li Chao, Shevchuk Andrew I, Stepanyan Ruben, Caldwell Matthew, Hughes Simon, Smart Trevor G, Gorelik Julia, Ostanin Victor P, Lab Max J, Moss Guy W J, Frolenkov Gregory I, Klenerman David, Korchev Yuri E
机构信息
Imperial College London, UK.
出版信息
Nat Methods. 2009 Apr;6(4):279-81. doi: 10.1038/nmeth.1306. Epub 2009 Mar 1.
Abstract
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.
摘要
我们描述了跳变模式扫描离子电导显微镜，它能够对活细胞复杂的三维表面进行非接触成像，分辨率优于20纳米。我们通过对培养的大鼠海马神经元网络和小鼠耳蜗毛细胞的机械感觉静纤毛进行成像，测试了该技术的有效性。该技术能够在生理条件下检测活细胞表面的纳米级现象。
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本文引用的文献
1
Towards nanomicrobiology using atomic force microscopy.
Nat Rev Microbiol. 2008 Sep;6(9):674-80. doi: 10.1038/nrmicro1948.
2
Nanoelectrochemistry of mammalian cells.
Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):443-8. doi: 10.1073/pnas.0711075105. Epub 2008 Jan 4.
3
Auditory mechanotransduction in the absence of functional myosin-XVa.
J Physiol. 2006 Nov 1;576(Pt 3):801-8. doi: 10.1113/jphysiol.2006.118547. Epub 2006 Sep 14.
4
Kinetics of electron-transfer reactions at nanoelectrodes.
Anal Chem. 2006 Sep 15;78(18):6526-34. doi: 10.1021/ac060924q.
5
Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy.
Angew Chem Int Ed Engl. 2006 Mar 27;45(14):2212-6. doi: 10.1002/anie.200503915.
6
Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system.
Biophys J. 2002 Dec;83(6):3296-303. doi: 10.1016/S0006-3495(02)75330-7.
7
Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: application to single virus-like particle entry into a cell.
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16018-23. doi: 10.1073/pnas.252458399. Epub 2002 Dec 4.
8
Lateral mechanical coupling of stereocilia in cochlear hair bundles.
Biophys J. 2001 Jun;80(6):2608-21. doi: 10.1016/s0006-3495(01)76231-5.
9
High-resolution structure of hair-cell tip links.
Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13336-41. doi: 10.1073/pnas.97.24.13336.
10
Functional localization of single active ion channels on the surface of a living cell.
Nat Cell Biol. 2000 Sep;2(9):616-9. doi: 10.1038/35023563.
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