Biology Department, Concordia University, Montreal, QC H4B1R6, Canada.
Plasmid. 2011 May;65(3):232-8. doi: 10.1016/j.plasmid.2011.02.005. Epub 2011 Mar 3.
Plasmid vectors using the Photorhabdus luminescenslux operon can be used for real time measurements of promoter activity. We have generated a series of lux vectors that have a conditional origin of replication, different selectable markers and the attP sequence from λ. Single copies of these plasmids can be integrated into the λ attachment site in the Escherichia coli chromosome. We constructed reporter derivatives and compared light production when the plasmids were present in multiple copies and in single copies. We also demonstrated that expression could be induced under the appropriate conditions.
利用发光杆菌 lux 操纵子的质粒载体可用于实时测量启动子活性。我们生成了一系列具有条件复制起点、不同选择标记和 λ 噬菌体 attP 序列的 lux 载体。这些质粒的单拷贝可以整合到大肠杆菌染色体的 λ 附着位点上。我们构建了报告基因衍生物，并比较了质粒在多拷贝和单拷贝时的发光情况。我们还证明了在适当的条件下可以诱导表达。
