Department of Chemistry & Key Laboratory of Analytical Sciences, College of Chemistry and Chemical engineering, Xiamen University, Xiamen 361005, China.
Talanta. 2011 Apr 15;84(2):287-92. doi: 10.1016/j.talanta.2011.01.019. Epub 2011 Jan 15.
Inductively coupled plasma mass spectrometry (ICP-MS) coupled on-line with an ion-pair reversed phase HPLC (IP-RP HPLC) was developed for determining the lability of Cd species. The IP-RP HPLC-ICP-MS system measures chromatographic behaviors of Cd species in the presence of different model complexing agents (L) with stability constants (logK(CdL)) from 3.8 to 19.0. Cd species with logK(CdL) higher than 16, between 8 and 16, and smaller than 8 was then classified into inert, moderately labile, and labile species, respectively. The conditional stability constants and dissociation rate constants were also estimated from their corresponding chromatographic behavior. This method was applied to evaluating the lability-dependent biouptake of different Cd species in Phaeodactylum tricornutum, a typical unicellular marine diatom. IP-RP HPLC-ICP-MS is a useful and promising technique for determining the lability of noncovalent-bonded metal species (such as Cd species) in the environment and for forecasting their corresponding bioavailability especially when their speciation cannot be rigorously controlled and measured.
电感耦合等离子体质谱（ICP-MS）与离子对反相高效液相色谱（IP-RP HPLC）在线联用，用于测定 Cd 物种的不稳定性。该 IP-RP HPLC-ICP-MS 系统在不同模型络合剂（L）存在下，测量 Cd 物种的色谱行为，稳定性常数（logK（CdL））为 3.8 至 19.0。logK（CdL）高于 16、在 8 到 16 之间和小于 8 的 Cd 物种分别被归类为惰性、中等不稳定和不稳定物种。还根据其相应的色谱行为估算了条件稳定常数和离解速率常数。该方法应用于评估不同 Cd 物种在典型的单细胞海洋硅藻三角褐指藻中的生物摄取的不稳定性依赖性。IP-RP HPLC-ICP-MS 是一种有用且有前途的技术，可用于测定环境中非共价键合金属物种（如 Cd 物种）的不稳定性，并预测其相应的生物利用度，特别是在不能严格控制和测量其形态的情况下。
