Katz P, Whalen G, Mitchell S R, Cupps T R, Evans M
Department of Medicine, Georgetown University School of Medicine, Washington, D.C.
Clin Immunol Immunopathol. 1990 Apr;55(1):148-55. doi: 10.1016/0090-1229(90)90076-3.
We examined the ability of human natural killer (NK) cells to modualte pokeweed mitogen (PWM)-induced polyclonal antibody production. Highly purified NK cells inhibited plaque-forming cell (PFC) responses and this suppression could be substantially increased by preincubation of NK cells with the known enhancers of NK cell lytic activity, interferon (IFN)-alpha, IFN-gamma, and interleukin-2. Additionally, costimulation of NK cells with two anti-CD2 antibodies (9-1 and 9.6), which recognize different epitopes on the CD2 molecule, also augmented the inhibitory effect. When subpopulations of NK cells were assayed for suppressor cell activity, this activity was primarily mediated by NK cells bearing Leu-7 but not Leu-2 (CD8) antigens. Thus, alteration of NK cell lytic activity may have significant effects on the immunoregulatory functions of these cells, which may have important implications for the in vivo manipulation of cytotoxic responses.
我们检测了人类自然杀伤（NK）细胞调节商陆丝裂原（PWM）诱导的多克隆抗体产生的能力。高度纯化的NK细胞抑制了空斑形成细胞（PFC）反应，并且通过用已知的NK细胞溶解活性增强剂α干扰素（IFN）、γ干扰素和白细胞介素-2对NK细胞进行预孵育，这种抑制作用可显著增强。此外，用两种识别CD2分子上不同表位的抗CD2抗体（9-1和9.6）对NK细胞进行共刺激，也增强了抑制作用。当检测NK细胞亚群的抑制细胞活性时，这种活性主要由带有Leu-7而非Leu-2（CD8）抗原的NK细胞介导。因此，NK细胞溶解活性的改变可能对这些细胞的免疫调节功能产生显著影响，这可能对体内细胞毒性反应的调控具有重要意义。
