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一种新型设备,可快速分离和鉴定稀有循环肿瘤细胞。

A new device for rapid isolation by size and characterization of rare circulating tumor cells.

机构信息

ScreenCell, 75012 Paris, France.

出版信息

Anticancer Res. 2011 Feb;31(2):427-41.

PMID:21378321
Abstract

BACKGROUND

Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells.

MATERIALS AND METHODS

The ScreenCell® devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size.

RESULTS

The ScreenCell® Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell® CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell® MB device filter.

CONCLUSION

Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell® devices may be able to simplify and improve non-invasive access to tumor cells.

摘要

背景

循环肿瘤细胞(CTCs)可能来源于原发性肿瘤中的克隆,这表明它们可以用于所有适用于原发性细胞的生物学检测。

材料和方法

ScreenCell® 设备是一种即用型、低成本的创新设备,它使用过滤器根据大小分离和分选肿瘤细胞。

结果

ScreenCell® Cyto 设备能够分离出罕见的、固定的肿瘤细胞,且具有较高的回收率。细胞在形态上得到很好的保存。免疫细胞化学和 FISH 检测可以直接在滤器上进行。ScreenCell® CC 设备可以分离出能够在培养中生长的活细胞。可以直接从 ScreenCell® MB 设备滤器上分离的肿瘤细胞中获得高质量的遗传物质。

结论

由于 ScreenCell® 设备体积小、用途广泛,并且能够在数分钟内分离 CTCs,因此它们可能能够简化和改善对肿瘤细胞的非侵入性获取。

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