非转移性结肠癌中循环肿瘤细胞的检出数量较低。
Low number of detectable circulating tumor cells in non-metastatic colon cancer.
机构信息
Department of Surgery, Roskilde Hospital, University of Copenhagen, Koegevej 7-13, DK-4000 Roskilde, Denmark.
出版信息
Anticancer Res. 2011 Feb;31(2):613-7.
BACKGROUND
The aim of the present study was to detect circulating tumor cells (CTCs) in the peripheral blood of patients with non-metastatic colon cancer and to evaluate whether there is a diurnal variation in the CTC counts. Furthermore, the study aimed to examine the correlation between CTCs and TNM stage, other paraclinical variables and prognosis.
PATIENTS AND METHODS
Blood samples were collected from 20 consecutive patients with colon cancer TNM stage I-III at four different perioperative time points. Detection of CTCs was performed using the immunological assay CellSearch®.
RESULTS
CTCs were detected in 1 out of 60 preoperative blood samples, resulting in a detection rate of 1 in 20 patients (5%; 95% confidence interval = 0.1-25%). None of the postoperative blood samples had CTC levels above the cut-off value (≥ 2 CTCs/7.5 ml blood).
CONCLUSION
The presence of CTCs in non-metastatic colon cancer is rare and barely detectable with the only commercially available assay for detection of CTCs, the CellSearch System.
背景
本研究的目的是检测非转移性结肠癌患者外周血中的循环肿瘤细胞（CTC），并评估 CTC 计数是否存在昼夜变化。此外，本研究旨在探讨 CTC 与 TNM 分期、其他临床相关变量和预后之间的相关性。
患者与方法
本研究共纳入 20 例连续的 TNM 分期为 I-III 期的结肠癌患者，在四个不同的围手术期时间点采集了血液样本。使用免疫分析试剂盒 CellSearch®检测 CTCs。
结果
术前的 60 份血样中，仅 1 份（1/60）检测到 CTC，检出率为 5%（95%置信区间为 0.1-25%）。术后的血样中均未检测到 CTC 水平超过检测限（≥2 CTCs/7.5 ml 血液）。
结论
唯一商业化的 CTC 检测试剂盒 CellSearch System 几乎检测不到非转移性结肠癌患者 CTC 的存在，提示 CTC 在非转移性结肠癌中较为罕见。
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