Wang-Yang M C, Buttke T M, Miller N W, Clem L W
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216.
Cell Immunol. 1990 Apr 1;126(2):354-66. doi: 10.1016/0008-8749(90)90327-n.
A low culture temperature of 27 degrees C inhibited mouse primary in vitro anti-hapten plaque-forming cell responses to a thymus-dependent (TD) antigen (Ag) (trinitrophenyl-keyhole limpet hemocyanin, TNP-KLH). In contrast, the magnitudes of secondary responses to TNP-KLH or primary responses to a thymus-independent (TI) Ag (TNP-lipopolysaccharide (LPS)) were unaffected. The low-temperature-sensitive step in the primary TD response occurred relatively early and preceded interleukin 2 (IL-2) secretion. Furthermore, the low-temperature-induced suppression could be obviated (rescued) by recombinant IL-2 or IL-4, but not by IL-1. Thus, the low temperature appeared to inhibit the function of virgin Th cells by preferentially affecting T cell-derived interleukin synthesis/secretion and not other cellular activities. These results also imply fundamental differences between the activation requirements of memory and virgin Th cells.
27摄氏度的低温抑制了小鼠体外对胸腺依赖性（TD）抗原（三硝基苯基-钥孔戚血蓝蛋白，TNP-KLH）的初次抗半抗原斑块形成细胞反应。相比之下，对TNP-KLH的二次反应幅度或对胸腺非依赖性（TI）抗原（TNP-脂多糖（LPS））的初次反应幅度不受影响。初次TD反应中对低温敏感的步骤发生得相对较早，且在白细胞介素2（IL-2）分泌之前。此外，重组IL-2或IL-4可消除（挽救）低温诱导的抑制作用，但IL-1不能。因此，低温似乎通过优先影响T细胞衍生的白细胞介素合成/分泌而非其他细胞活动来抑制处女型Th细胞的功能。这些结果也暗示了记忆性和处女型Th细胞激活要求之间的根本差异。
