Netto Jeremy D, Musk Gabrielle C, Maker Garth L, Trengove Robert D
Separation Science and Metabolomics Laboratory, Murdoch University, Western Australia, Australia.
Biomed Chromatogr. 2011 Dec;25(12):1374-80. doi: 10.1002/bmc.1613. Epub 2011 Mar 4.
Ketamine and medetomidine are commonly combined to sedate or anaesthetize a wide range of animal species. Despite this, there are few methods for the simultaneous quantitative analysis of the two drugs. This study describes the use of solid-phase extraction sample preparation followed by liquid chromatography-tandem mass spectrometry for the quantitative analysis of both drugs in ovine plasma. Extraction recovery was 93% for ketamine and 95% for medetomidine. The lowest limit of detection for ketamine was 1 ng/mL and for medetomidine 2 ng/mL, with linearity greater than 0.99 for both. Intra-day and inter-day precisions for both drugs were less than 10 and 7%, respectively. Application of the method to samples obtained from pregnant ewes and their fetuses showed placental transfer of the drugs over time such that there was no significant difference in plasma concentration at delivery. In summary, a validated method has been developed for the simultaneous quantification of ketamine and medetomidine in ovine plasma samples which can be used to study the pharmacokinetics of these drugs.
氯胺酮和美托咪定通常联合使用，用于多种动物的镇静或麻醉。尽管如此，同时对这两种药物进行定量分析的方法却很少。本研究描述了采用固相萃取样品制备，随后进行液相色谱 - 串联质谱法对绵羊血浆中的这两种药物进行定量分析。氯胺酮的萃取回收率为93%，美托咪定的萃取回收率为95%。氯胺酮的最低检测限为1 ng/mL，美托咪定的最低检测限为2 ng/mL，两者的线性均大于0.99。两种药物的日内和日间精密度分别小于10%和7%。将该方法应用于从怀孕母羊及其胎儿获取的样本，结果显示随着时间推移药物会发生胎盘转运，因此分娩时血浆浓度无显著差异。总之，已开发出一种经过验证的方法，用于同时定量绵羊血浆样本中的氯胺酮和美托咪定，该方法可用于研究这些药物的药代动力学。
