Department of Pharmacology, New York University School of Medicine and NYU Cancer Institute, New York, NY, USA.
Wiley Interdiscip Rev Syst Biol Med. 2011 Nov-Dec;3(6):649-65. doi: 10.1002/wsbm.140. Epub 2011 Mar 4.
The recent development of high-throughput sequencing technologies and the availability of whole genome sequences of a variety of living organisms, including that of humans, have led to an enormous push in the quest for a comprehensive inquiry for the function of each and every gene discovered in different model organisms. A major conclusion from the sequencing projects was that while forward genetics had been extremely successful in identifying key genes/components of many biological processes, such as signal transduction cascades, the function(s) of the majority of genes in the genome remains a mystery. In this article, we discuss the use of a variety of high-throughput postgenomic tools, including functional genomics, proteomics, and chemical genetics that are being implemented in an exhaustive molecular dissection of a key evolutionarily conserved signal transduction pathway, namely the Wnt/wingless (wg) pathway and its associated signaling network.
近年来高通量测序技术的发展以及各种生物（包括人类）的全基因组序列的可用性，推动了人们对每个发现于不同模式生物的基因的功能进行全面探究。测序项目的一个主要结论是，虽然正向遗传学在识别许多生物过程（如信号转导级联）的关键基因/成分方面非常成功，但基因组中大多数基因的功能仍然是个谜。在本文中，我们讨论了使用各种高通量后基因组工具，包括功能基因组学、蛋白质组学和化学遗传学，对一个关键的进化保守信号转导途径（即 Wnt/wingless [wg] 途径及其相关信号网络）进行详尽的分子剖析。
