Vasil'eva G I, Ivanova I A, Tiukavkina S Iu, Bespalova I A, Omel'chenko N D, Doroshenko E P
Zh Mikrobiol Epidemiol Immunobiol. 2010 Nov-Dec(6):58-61.
To study effect of neutrophilokines on functional activity of macrophages (Mph) during formation of immunity against cholera.
In order to obtain peritoneal neutrophils (Nph), 2 ml of 0.1% glycogen solution in buffered with phosphates sodium chloride solution was administered intraperitoneally to 100 outbred mice. Vibrio cholerae 1130 in dose 10 microbial cells/Nph and cholera toxin (CT) in dose 1 or 10 mcg/ ml were used as inducers of neutrophilokines synthesis. Obtained neutrophilokines were assessed on their effect on phagocytic activity of Mph, resistance of these cells to cytotoxic and apoptogenic effects of Vibrio cholerae and CT as well as effect on lysosomal apparatus of Mph.
It was established that neutrophilokines induced by Vibrio cholerae and CT stimulate killer activity of Mph and lability of their lysosomal membranes, and suppress programmed death of these cells.
Results of studies revealed immunoregulatory activity of neutrophilokines relative to Mph and demonstrated ability for cooperation between mono- and polynuclear phagocytes mediated by cytokines and, in particular, neutrophilokines.
研究嗜中性粒细胞因子在霍乱免疫形成过程中对巨噬细胞（Mph）功能活性的影响。
为获取腹腔嗜中性粒细胞（Nph），向100只远交系小鼠腹腔注射2 ml用磷酸盐缓冲的0.1%糖原氯化钠溶液。将剂量为10个微生物细胞/Nph的霍乱弧菌1130以及剂量为1或10 mcg/ml的霍乱毒素（CT）用作嗜中性粒细胞因子合成的诱导剂。评估所获得的嗜中性粒细胞因子对Mph吞噬活性的影响、这些细胞对霍乱弧菌和CT的细胞毒性及凋亡诱导作用的抗性以及对Mph溶酶体装置的影响。
确定由霍乱弧菌和CT诱导产生的嗜中性粒细胞因子可刺激Mph的杀伤活性及其溶酶体膜的不稳定性，并抑制这些细胞的程序性死亡。
研究结果揭示了嗜中性粒细胞因子相对于Mph的免疫调节活性，并证明了由细胞因子，特别是嗜中性粒细胞因子介导的单核和多核吞噬细胞之间的合作能力。
