[从支气管肺部疾病患儿分离出的不可分型流感嗜血杆菌脂寡糖的分型]
[Subtyping of lipooligosaccharides of non-typeable haemophilus influenzae isolated from children with bronchopulmonary diseases].
作者信息
Vaneeva N P, Novikova O V, Orlova O E, Kalina N G, Elkina S I, Tokarskaia M M, Kotosova L K, Iastrebova N E
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2010 Nov-Dec(6):93-5.
AIM
Subtyping of lipooligosaccharides (LOS) of non-typeable strains of Haemophilus influenzae (NTHi) isolated from children with bronchopulmonary diseases.
MATERIALS AND METHODS
Lipooligosaccharides obtained from 62 acapsular strains of H. influenzae were studied by vertical SDS-electrophoresis in PAAG.
RESULTS
Majority of LOS formed electrophoretically mobile components in low molecular mass zone. Obtained results allowed to differentiate 23 subtypes of LOS. Lipooligosaccharides of majority of strains (67.7%) belonged to one of 10 main subtypes, 30.6% of strains belonged to mixed subtypes because they had signs of 2-3 subtypes.
CONCLUSION
Strains possessing LOS of three subtypes--VI, VII, and X--were significantly more prevalent in pediatric patients (p < 0.05). More than one third (43.5%) of studied NTHi strains belonged to these subtypes.
目的
对从患有支气管肺部疾病的儿童中分离出的不可分型流感嗜血杆菌（NTHi）的脂寡糖（LOS）进行分型。
材料与方法
通过在聚丙烯酰胺凝胶中的垂直SDS电泳对从62株无荚膜流感嗜血杆菌中获得的脂寡糖进行研究。
结果
大多数LOS在低分子量区域形成电泳可移动成分。所获结果可区分出23种LOS亚型。大多数菌株（67.7%）的脂寡糖属于10种主要亚型之一，30.6%的菌株属于混合亚型，因为它们具有2 - 3种亚型的特征。
结论
拥有VI、VII和X三种亚型LOS的菌株在儿科患者中显著更为常见（p < 0.05）。超过三分之一（43.5%）的研究NTHi菌株属于这些亚型。
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