Inzana T J
J Infect Dis. 1983 Sep;148(3):492-9. doi: 10.1093/infdis/148.3.492.
Lipopolysaccharide (LPS) was extracted from 50 isolates of Haemophilus influenzae by a rapid micromethod and was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. LPS isolated by this method was electrophoretically indistinguishable from conventionally purified LPS but lacked the large degree of heterogeneity indicative of variable polymerization of O-side-chain repeating units; one to four bands appeared in gels. Electrophoretic profiles of LPS from H influenzae type b varied among strains and were useful for subtyping. Thirty-three isolates of type b were classified into 11 subtypes on the basis of stable band mobilities. Certain isolates of identical outer-membrane-protein subtype differed in LPS subtype. The LPS profiles of non-b, typable strains of H influenzae were generally similar in microheterogeneity to those of type b strains, while those of untypable isolates usually displayed less electrophoretic variation.
采用快速微量法从50株流感嗜血杆菌中提取脂多糖（LPS），并通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和银染进行分析。用该方法分离得到的LPS在电泳上与传统纯化的LPS无法区分，但缺乏O侧链重复单元可变聚合所指示的高度异质性；凝胶中出现一至四条带。b型流感嗜血杆菌LPS的电泳图谱在菌株间存在差异，可用于亚型分析。根据稳定的条带迁移率，33株b型菌株被分为11个亚型。某些外膜蛋白亚型相同的菌株在LPS亚型上存在差异。非b型、可分型的流感嗜血杆菌菌株的LPS图谱在微异质性方面通常与b型菌株相似，而不可分型菌株的LPS图谱通常显示出较少的电泳变异。
