Institute for Organic Chemistry, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
J Org Chem. 2011 Apr 1;76(7):2301-4. doi: 10.1021/jo102519k. Epub 2011 Mar 7.
4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) as a fluorescent label can be incorporated into DNA by two conceptually different ways: the non-nucleosidic DNA base surrogate Bo exhibits high brightness but no preferential base-pairing properties, whereas the BODIPY-modified uridine BodU has reduced quantum yields but shows preferred Watson-Crick base pairing with adenine.
4,4-二氟-4-硼-3a,4a-二氮杂-s-茚并（BODIPY）作为荧光标记物，可以通过两种不同的概念方式被整合到 DNA 中：非核苷类 DNA 碱基类似物 Bo 具有高亮度但没有优先碱基配对特性，而 BODIPY 修饰的尿嘧啶 BodU 量子产率降低，但与腺嘌呤显示出优先的沃森-克里克碱基配对。
