Imamura Takashi, Sato Maki, Hashimoto Koichi, Ishii Tsutomu, Goto Aya, Go Hayato, Kawarada Tsutomu, Kawasaki Yukihiko, Momoi Nobuo, Ujiie Niro, Fujimori Keiya, Hosoya Mitsuaki
Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima City, Fukushima, Japan.
J Matern Fetal Neonatal Med. 2011 Nov;24(11):1312-6. doi: 10.3109/14767058.2010.548885. Epub 2011 Mar 7.
To examine the expression levels of glucocorticoid receptor (GR) isoforms in peripheral blood mononuclear cells (PBMCs) and serum cortisol levels in cord blood from term infants.
The study population consisted of 172 term infants who were delivered from healthy pregnant women. GRalpha and GRbeta expression levels, and serum cortisol level in cord blood were determined by real-time PCR and ELISA, respectively.
Detection rates of GRalpha, GRbeta, and GAPDH were 100%, 63.4%, and 100%, respectively. The expression level of GRalpha was about 200 times that of GRbeta. There were no associations between GR expression level and clinical variables. There were significant associations of low UmApH, maternal gravidity or parity, and vaginal delivery with a high cortisol level; however, there were no correlations between GR expression levels and cortisol level.
It is considered that glucocorticoid effects could be expected from the fetal period to the neonatal period, because GRalpha expression level was not related to perinatal factors, GRbeta expression level, and cortisol level in term infants. Further studies of larger populations including very preterm and small for gestational age infants are necessary to determine the balance of expression between GRalpha and GRbeta, and cortisol level.
检测足月儿脐血中糖皮质激素受体（GR）亚型在外周血单个核细胞（PBMCs）中的表达水平及血清皮质醇水平。
研究对象为172例由健康孕妇分娩的足月儿。分别采用实时荧光定量PCR和酶联免疫吸附测定法检测脐血中GRα和GRβ的表达水平以及血清皮质醇水平。
GRα、GRβ和甘油醛-3-磷酸脱氢酶（GAPDH）的检出率分别为100%、63.4%和100%。GRα的表达水平约为GRβ的200倍。GR表达水平与临床变量之间无相关性。脐动脉血pH值低、母亲妊娠次数或产次以及阴道分娩与高皮质醇水平之间存在显著相关性；然而，GR表达水平与皮质醇水平之间无相关性。
由于足月儿GRα表达水平与围生期因素、GRβ表达水平及皮质醇水平无关，因此认为从胎儿期到新生儿期均可预期糖皮质激素的作用。有必要对包括极早产儿和小于胎龄儿在内的更大样本进行进一步研究，以确定GRα和GRβ表达平衡以及皮质醇水平。
